rabbit anti ampar  (Danaher Inc)

Journal: Pharmaceuticals

Article Title: Anti-AMPA Receptor Autoantibodies Reduce Excitatory Currents in Rat Hippocampal Neurons

doi: 10.3390/ph16010077

Figure Lengend Snippet: Rabbit anti-AMPAR GluR3B immunogenicity response. ( A ) ELISA of protein A-purified AMPAR (GluR3) Aabs against the GluR3 immunisation peptide or an irrelevant peptide; n = 3 technical replicates. ( B ) Western blot of mouse whole brain lysate probed with a commercial anti-AMPAR antibody (cAMPAR), anti-AMPAR Aabs, a class-specific negative control rIgG (naïve) or secondary antibody only (negative control). Representative blots from n = 3 technical replicates. ( C ) Immunocytochemical staining of fixed primary cortical neuron on cultures. Cells (DIV8) were stained with anti-AMPAR Aabs (red), anti-βIII tubulin (green), GFAP (white) and nuclei counterstained with DAPI (blue). Examples of labelling of hippocampal neurons with anti-AMPAR Aabs (red) is indicated by the white arrows. Scale bars = 20 μm. Representative images from n = 3 technical replicates.

Article Snippet: Primary and secondary antibodies used were as follows: rabbit anti-AMPAR (1:100; raised against residues 60–73 of rat GluR3 ATD, AGC-010, Alomone Labs, Jerusalem, Israel); rabbit anti-IgG 1 (rIgG, 1:100, 011-000-003, Jackson ImmunoResearch, Cambridge, UK); mouse anti-IgG 2b (1:100, 70–4732, BioLegend, London, UK); mouse anti-βIII-tubulin (mIgG2b, 1:500, 801201, BioLegend, London, UK); mouse anti-glial fibrillary acidic protein (GFAP) (1:400, MAB3402, Millipore); goat anti-rabbit or anti-mouse Alexa Fluor 488/594/647 (all at 1:1000, Life Technologies, Loughborough, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Purification, Western Blot, Negative Control, Staining

Journal: Heliyon

Article Title: NBQX mediates ventricular fibrillation susceptibility in rat models of anxiety via the Nrf2/HO-1 pathway

doi: 10.1016/j.heliyon.2024.e37358

Figure Lengend Snippet: NBQX enhanced the expression of CX43 and ion channel proteins. (A) Representative immunoblotting images of protein CX43, KV4.2, KV4.3, AMPAR. (B–E) Immunoblotting quantitative analysis of KV4.2, KV4.3, CX43, and AMPAR expression（n = 3 per group）. (F, G) Typical images of CX43 in immunohistochemical staining and quantitative analysis of the CX43 area; scale bar = 50 μm (n = 5 in each group). (H, I) Immunofluorescence staining and quantitative analysis of CX43 expression; scale bar = 50 μm (n = 5 per group)., **p < 0.01, ***p < 0.001.

Article Snippet: The primary antibodies used for western blotting analysis included AMPAR (1:1000 dilution; #4676; Cell Signaling Technology), Connexin 43 (CX43,1:1000 dilution; #3512; Cell Signaling Technology), KV4.2 (1:1000 dilution; #74748; Cell Signaling Technology), KV4.3 (1:1000 dilution; bs-3762R; Bioss), collagen I (1:5000 dilution; 67288; Proteintech), collagen III (1:1000 dilution; 22734; Proteintech), Nrf2 (1:2000 dilution; 16396; Proteintech), HO-1 (1:1000 dilution; 10701; Proteintech), Bcl2 (1:1000 dilution; #4223; Cell Signaling Technology), BAX (1:1000 dilution; #5023; Cell Signaling Technology), Caspase-3 (1:1000 dilution; #14220; Cell Signaling Technology) and GAPDH (1:4000 dilution; 60004; Proteintech).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Staining, Immunofluorescence